Mayor Kevin Cranston signed the 90in high card which is touring the county thanks to the Allied Rapid Reaction Corps (ARRC).
The card began travelling on April 21 and will be on tour for a month. It will be displayed on June 9, her official birthday weekend, at the Imjin Music Festival 2016 at Imjin Barracks before being sent to Her Majesty.
“I was pleased to sign the birthday card to Her Majesty the Queen on behalf of Stroud,” said Mayor Kevin Cranston.
“She has devoted her life to the service of Britain and the Commonwealth and the country owes her a huge thank you.”
The card was designed locally by the Bigger Printing Company and the artwork created by children at the Churchdown Village Junior School.
To learn more about the music festival and get your free tickets, go to www.imjinmusicfestival.uk.