In a unanimous vote, councillors supported a proposal by mayor John Marjoram to ban the cull.
It follows an appeal by Stroud 100, a local group opposed to the culling of badgers in Stroud.
The group has 82 people and organisations signed up, including farmers and businesses, with more than 1,200 acres.
West Gloucestershire and West Somerset have been chosen by DEFRA as the most suitable areas to pilot controlled shooting of badgers.
It is currently tendering for marksmen to carry out the cull, in a bid to cut the cases of bovine tuberculosis.
Councillor Kevin Cranston said: “My understanding of TB in cattle is that it is largely the result of intensive farming and over use of antibiotics and growth hormones; organic and biodynamic herds have much lower rates of infection. The idea of killing badgers is the wrong solution.”
The ban will affect land owned by the town council including Uplands Fields, Park Gardens, the Long Ground, and the allotments sites in Summer Street, Uplands, Gannicox, Spider Lane and Bisley Old Road.