Developer Charles Church wants to build in Grange Fields, Wick Street.
Town councillors believe that the proposal would cause harm to the Area of Outstanding Natural Beauty and would be detrimental to wildlife, particularly the protected species on the site.
“The Town Council’s view is that it cannot possibly be right to alleviate any temporary perceived deficiency in housing supply by allowing permanent development that would harm the natural beauty of the area and the AONB,” states the council.
Councillors also have concerns about increased congestion on the A46 and that no long-term employment is provided by proposed development.
Councillor Simon Arundel, chair of the consultations committee, said councillors felt that the development would detract from the gateway to the town.
“A gateway to a town is provided to welcome visitors and promote the town. It contributes to a sense of place both in the buildings and the space and how they integrate with the surroundings,” said Councillor Arundel.
“This development would harm rather than enhance the gateway to the town.”
In its objections, the council states: “The Town Council does not believe that this site is needed to make good any shortfall in housing land.
“Even if it were, the harm caused would mean that other sites should be considered before this one.”
Natural England, Cotswold Conservation Board and the Campaign for the Protection of Rural England have also objected to the plans. More than 500 members of the public have also objected to the proposal.
Stroud District Council is due to consider the application in July.